Oyster mushroom cultivation technic II

In Indonesia.

There is considerable practical technology for cultivation of oyster mushroom Pleurotus spp, which stages make media spawn (spawn) and resistance produce oyster mushrooms. In the prisoners make spawn media there are 10 steps that need to be done. First, the media materials in the form of grain or a mixture of sawdust sawn albusia (SKG) plus millet seeds 1 (42%): 1 (42%). This raw material is the best.

The second step, the raw materials are washed and boiled for 30 minutes using a pressure cooker or saucepan. The third step, the raw material was drained with a sieve. Add 1% lime (CaCl3), 1% gypsum (CaSO4), vitamin B complex (very little) and or 15 percent bran. The water content of 45-60% with the addition of a little water and pH 7.

The fourth step, the raw material is then distributed to the baglog polypropylene or a bottle of milk or bottle at the same day. Perbotol 50-60% filled seed media, corked cotton / kapok, wrapped in newspaper / aluminum foil. The fifth step, in autoclav sterilization or pasteurization for 2 hours 8 hours on the same day. Autoclave temperature 121 degrees C, pressure of 1 lb, for 2 hours. Pasteurization temperature 95 degrees C.

The sixth step, do the inoculation with laminar flow a day later. After the seed medium temperature down to room temperature conducted inoculation of seedlings from pure cultures on PDA (mycelium colony of 2-3 seeds per bottle). Seventh step, incubation (15-21 days of mycelial growth) in the incubation chamber / incubator, temperature of 22-28 degrees C.

Step eight, bottles or the contents of seedlings baglog whipped every day, two to three times. This is done for the growth of fungal mycelium seeds evenly and quickly and the media does not clot seeds / harden. Ninth, mushroom mycelium spawn filled with fungal mycelium growth characteristics of a compact and uniform.

The final step, the fungus is used as an inoculant / seed the parent / healthy seed multiplication to 1 and 2. Seeds are stored in a refrigerator for 1 year, if not immediately used.

The next stage is the production of oyster mushrooms (Pleurotus spp.) In this stage there are 10 steps. First, prepare lumber treated with wood dust. Soak for 0-12 hours (depending on the species / strain used sawdust). The second step, drain until no water, on the same day using a wire strainer or wire strainer.

The third step, making the substrate / growing medium, on the same day. Add 5-15% bran or polar (depending on the species / strain used), 2% lime (CaCO3), 2% gypsum (CaSO4) and water, stirring evenly, 65% substrate moisture content, pH 7.

The fourth step, distributed into polypropylene at ahri baglog that too. In the container compact enough, give the center hole, fitted mouth pralon ring, then covered with cotton / wax paper. The fifth step, sterilization / pasteurization, one day later. Store in the steam room or steam in the drum with the media in the baglog temperature 95-120 degrees C for 1-3 times 8 hours depending on the amount of substrate that will be pasteurized. The sixth step, the substrate inoculation inoculation with spawn in the room. Setelahsuhu baglog substrate down to room temperature, inokulasikan seeds on the substrate in a laminar flow. Seeds 10-15gr/kg substrate.

Seventh step, incubation baglog substrate (mycelium growth 15-30 days). Houses mushroom / lemur / incubation chamber kept dry and clean, the temperature of 22-28 degrees C without light. Step eighth, opened the ring opened baglog substrate (7-15 days later). How to open vary, depending on the type of wood used mushrooms.

Step nine, baglog arranged on shelves in houses fungus (mushroom growth of 10-15 days later, grew pin head / will grow fruit). Will grow the fruit is drenched with clean water for fungal growth. For oyster mushrooms, which doused the house mushrooms. For watering mushroom directly on the substrate until soaked. Mushroom house temperature 16-22 degrees C RH: 80-90%.

The final step oyster mushroom harvest / ear. Harvest of less than 9 times in less than 1.5 months depending on how the maintenance / watering mushrooms and cleanliness lemur. Or the rest of the harvest 2-5 times a week.

Important factors to be considered in this oyster mushroom cultivation is a matter of hygiene, application of quality seeds, advanced seed production (pure culture, spawn, seed spread), production technology, the growing medium / substrate and maintenance as well as how to harvest oyster mushrooms.